Effect of liganded hemoglobin S and hemoglobin A on the aggregation of deoxy-hemoglobin S.
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چکیده
منابع مشابه
Aggregation of hemoglobin S and hemoglobin CHarlem with nonsickle hemoglobin in concentrated phosphate buffer.
The effect of HbA and HbF on both the aggregation and deaggregation of deoxy HbS and deoxy HbCHarlem in concentrated phosphate buffers was studied by a turbidimetric method. Although pure deoxy HbA is fully soluble in 2.4 M potassium phosphate buffer, the same concentration of deoxy HbAS was insoluble in the same buffer. Since there was little dissolved hemoglobin in the solute of a mixture of ...
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ical goal for imprecision (CV) was 13.6% [3]. Thus the performances of all of the methods may be satisfactory. However, as Fraser and Peterson have argued, even when biological variation, not analytical variation, is the limiting source of variation in a test, there is still great advantage in using a method with the least possible imprecision and bias [4]. The aca V was the least precise instr...
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Recent interest in therapies for sickle cell anemia based on elevating fetal Hb has made accurate estimates of the sparing effect of fetal Hb (Hb F) and other non-sickle Hbs on sickle Hb (Hb S) polymerization essential. We have developed a technique, using HbCO as surrogate for HbO2, that enables us to assess the solubility of Hb S as a function of ligand saturation under conditions that mimic ...
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We previously reported that circulating hemoglobin (Hb) CC erythrocytes contain oxygenated HbC crystals with little or no HbF and that HbF inhibits in vitro crystallization of HbC. We now report that HbS accelerates in vitro crystallization of HbC. Crystals were formed in 1.8 mol/L potassium phosphate buffer, pH 7.4, at 30 degrees C and were counted in several time intervals with a hematocytome...
متن کاملLow proportions of glycosylated hemoglobin associated with hemoglobin S and hemoglobin C.
Using a cation-exchange chromatographic method, we found normal or subnormal values for glycosylated hemoglobin in a few diabetic patients with persistent hyperglycemia. Subsequent investigations revealed that these unexpected results had originated from black patients with diabetes. In view of common occurrence of abnormal hemoglobins in the Negro population, we subjected blood preparations to...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)83840-1